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Phylo-Win
Graphical color interface for molecular phylogenetic inference. It performs neighbor-joining, parsimony, and maximum likelihood methods and bootstrap with any of them.
http://pbil.univ-lyon1.fr/software/phylowin.html
Univ-lyon1.fr ~
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Mesquite
Experimental, modular software for evolutionary biology, designed to help biologists analyze comparative data about organisms.
http://mesquiteproject.org/mesquite/mesquite.html
Mesquiteproject.org ~
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PAUP*
PAUP is the most widely used package for phylogenetic inference, and now implements maximum likelihood and distance methods as well as various forms of parsimony. Powerful and very easy to use, particularly the Mac version.
http://paup.csit.fsu.edu/
Fsu.edu ~
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r8s
Open source software for estimating absolute rates of molecular evolution and divergence times on phylogenetic trees.
http://loco.biosci.arizona.edu/r8s/
Arizona.edu ~
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